Peripheral Nerve

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A histological slide of a peripheral nerve stained to highlight its structural components. In the transverse section, multiple circular axons are visible, each encased by a myelin sheath, creating a distinctive ring-like appearance. The perineurium, a connective tissue layer, surrounds groups of axons, forming fascicles. In the longitudinal section, nerve fibers appear as elongated, wavy structures running parallel to each other. Schwann cells are present along the axons, and nodes of Ranvier, where the myelin sheath is interrupted, can be seen as small gaps along the fibers
Figure 1: A histological slide of a peripheral nerve stained to highlight its structural components.

In the transverse section, multiple circular axons are visible, each encased by a myelin sheath, creating a distinctive ring-like appearance. The perineurium, a connective tissue layer, surrounds groups of axons, forming fascicles. In the longitudinal section, nerve fibers appear as elongated, wavy structures running parallel to each other. Schwann cells are present along the axons, and nodes of Ranvier, where the myelin sheath is interrupted, can be seen as small gaps along the fibers

Only the axons of these neurons are in the nerve. We name them “fibers” when we view them in a nerve.  So, a nerve is essentially bundles of little cylinders (axons).  These axons can be myelinate or not and they can be sensory or motor.  For example, the sciatic nerve carries motor information to muscles in your legs. It also collects sensory information from sensory receptors in your legs.  

The picture here shows a the cross section of a nerve.  On the outer, right side portion, you can see little circles, which are all myelinated axons cut transversely.  The circles have a dark pink outline that is the endoneurium, wrapping each axon.  Inside the circle seems to be filled with tissue that does not take a stain and is a white color.  This is the myelin that does not like to take up this H&E stain.  However, if you inspect closely, you will see a faint pink dot. It is in the center of each of those white circles.  This is the axon itself.

Sweeping across the center of the nerve are axon cut in something like an oblique section.  The axons appear as faint pink lines instead of dots. You are almost able to see some nodes of Ranvier. The myelin is absent along the axon in these areas.

Histological slide of a peripheral nerve showing axons in both transverse and longitudinal sections. The transverse section displays round axons surrounded by myelin sheaths, forming a honeycomb-like pattern. In the longitudinal section, axons appear as elongated fibers running parallel, with visible nodes of Ranvier between Schwann cells
Figure 2 Histological slide of a peripheral nerve showing axons in both transverse and longitudinal sections. The transverse section displays round axons surrounded by myelin sheaths, forming a honeycomb-like pattern. In the longitudinal section, axons appear as elongated fibers running parallel, with visible nodes of Ranvier between Schwann cells.
Histological slide of a peripheral nerve showing a mix of longitudinally and transversely sectioned axons with pink-stained connective tissue.
Figure 3: Histological section of a peripheral nerve showing longitudinally cut axons on the left and transversely cut axons on the right, with visible Schwann cell nuclei and myelin sheaths.
High-power magnification histological slide of a longitudinally cut nerve, stained pink. Wavy, elongated nerve fibers run parallel, with visible nodes of Ranvier appearing as small gaps between segments of myelin. The delicate endoneurium surrounds individual axons, while Schwann cells may be seen along the fibers
Figure 4 High-power magnification histological slide of a longitudinally cut nerve, stained pink. Wavy, elongated nerve fibers run parallel, with visible nodes of Ranvier appearing as small gaps between segments of myelin. The delicate endoneurium surrounds individual axons, while Schwann cells may be seen along the fibers

Figure 1: Parotid gland, H&E, 40X Slide 180-1
Figures 2, 3 and 4 Peripheral myelinated nerve, cross section, 40X  Slide 68
All by University of Michigan Histology, licensed under CC BY-NC-SA 3.0.

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