Vagina

The vagina is a fibromuscular tube that serves as the passageway between the uterus and the external genitalia. Histologically, it is lined by a non-keratinized stratified squamous epithelium, which provides protection against friction and potential pathogens. Notably, the vaginal epithelium responds to estrogen by accumulating glycogen, which is metabolized by resident bacteria (primarily Lactobacillus) into lactic acid. This process maintains the acidic pH of the vaginal environment, helping to prevent infections.

Light microscopy image of a vagina slide displaying pink stained stratified squamous epithelium and blue stained connective tissue. The stratified squamous epithelium covers the superficial layer. The connective tissue is located in the deep layer.
Vagina slide displaying tissue structure.

The deeper layers of the vaginal wall include a muscularis layer, composed of inner circular and outer longitudinal layers of smooth muscle that allow for distensibility during intercourse and childbirth. The outermost layer is the adventitia, a connective tissue layer that anchors the vagina to surrounding pelvic structures and contains additional blood vessels, lymphatics, and nerves.

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