Pl. II · Epididymis

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Pl. II · FAM. MATURANTIDAE

Maturans stereociliatus

The Long Wait  ·  epididymis

The plate

The specimen, drawn

Pl. II

Hand-drawn field-guide plate headed Maturans stereociliatus, var. gordius — The Long Wait, Pl. II, FAM. MATURANTIDAE. A single brown thread-like worm is wound into a loose tangle filling the upper half, its loops crossing over and under one another, with one free end led out to the lower left and the other to the upper right. Faint slate water lines run behind it. Below, a pink schematic of the epididymal wall: a pseudostratified lining with nuclei at several heights, a dense fringe of long leaning stereocilia along its upper surface, a thin strip of smooth muscle beneath, and a lumen above packed with small comma-shaped sperm nuclei. A circular inset at ×400 shows the same lining enlarged.

The worm as it was found, knotted, with the epididymal wall laid out flat below it and the stereocilia read at ×400 in the circle.

Habitat & range

Where it lives

Body map

Body map: a male human figure drawn as a broken pencil outline with diagonal hatching inside it, on a transparent ground. Numbered cream markers outlined in rust mark the epididymis on the back of each testis. Below the figure, a heading reading "where it lives" and a numbered key naming each marker.

A single coiled duct lying along the back of each testis, inside the scrotum.

Range

  • On the posterior surface of the testis, inside the scrotum — head, body and tail, in that order.
  • About 5 cm from end to end; uncoiled, the duct runs to roughly six metres.
  • Sperm spend somewhere between two days and two weeks in it, and most of that in the tail.
  • They arrive unable to swim and leave able to. Nothing else in the tract does that job.

Collection record

  • Add your slide numbers here.

Niche

What it does for a living

Finish what the testis started — reabsorb most of the fluid the sperm arrived in, add the secretions that make them motile and able to fertilise, and then hold the finished product until it is called for.

field collected specimen

How to know it on sight

  • Roughly circular tubule profiles, close together, each one a section through the same enormously long duct.
  • A tall pseudostratified columnar lining whose nuclei sit at two or three different heights, so it looks layered and is not.
  • Long tufted projections reaching into the lumen — stereocilia. They are microvilli and they do not move.
  • A thin coat of smooth muscle outside. Thin is the point: compare the wall on Pl. III.
Histological light microscopy image of the epididymis. Displaying a roughly circular epididymal tubule lined by pseudostratified columnar epithelium. The periphery is surrounded by connective tissue with scattered nuclei and blood vessels. The tubule's lumen has a cell accumulation. pink and purple staining is visible.

Specimen 1

A histology slide displaying the epididymis. The image consists of numerous tubules cut in cross-section, revealing circular and oval structures. The staining creates a two-toned effect, with a darker pink/red outlining the epithelial cells and lighter, mostly clear lumen. Connective tissue is lightly stained. The tubules are densely packed across the frame.

Tubules in cross-section, the epithelium picked out in darker pink against lighter, mostly clear lumina.

Specimen 2

Histological section: Testis (top) and epididymis (bottom). Testis: densely packed seminiferous tubules with pink-stained basement membranes and lightly stained lumens. Epididymis: coiled tubules with more defined lumens and stroma. Hematoxylin and eosin stain. Bright field microscopy, low magnification.

Testis above, epididymis below, on one slide. The wall changes character completely across that boundary.

Specimen 3

Micrograph of pseudostratified ciliated columnar epithelium, stained reddish-pink. The image displays closely packed, multi-layered cells with dark nuclei along the bottom edge. Cilia, visible as short, fine projections, line the top surface. White spaces represent lumens. The composition is tightly cropped, with high detail.

The lining at high power. Pseudostratified columnar, nuclei at several levels, and a dense apical fringe.

Hand-drawn teaching figure in coloured pencil, pink tissue with plum nuclei on cream: three labelled pairs. Epididymis against ductus deferens, the first with tall stereocilia and thin muscle, the second with short stereocilia and a huge muscular wall. Prostate against seminal vesicle, separate irregular pockets in muscular stroma against one lumen folded into a honeycomb. Proliferative against secretory endometrium, straight narrow glands in a thin layer against coiled saw-toothed glands with deep spiral arteries.

People keep calling her by the wrong name

Look-alikes

Two plates, and the first is the single most-missed pair in this volume. The ductus deferens shares this lining exactly; only the wall tells them apart. The second is the gonad upstream, where the cells in the wall are the product rather than the plumbing.

Ductus deferens

Light micrograph of a ductus deferens, stained pink. The central lumen is small and irregularly shaped. The muscularis externa forms a thick, circular structure. The surrounding tissue consists of connective tissue and other structures from the spermatic cord. The image is evenly illuminated.
Ductus deferens — a pinhole lumen in an enormous wall.

Same lining family, shorter stereocilia, and a muscularis out of all proportion to the lumen. Muscle-to-lumen ratio decides it.

Seminiferous tubule

High-magnification photomicrograph of seminiferous tubules. The image displays a field of round to irregular tubular structures in cross-section, stained with a pink/purple dye providing contrast to cell nuclei. The tubular walls are composed of densely packed cells, and the hollow centers are clear due to the dye's absence. The tubules are oriented in various directions, filling the frame entirely.
Seminiferous tubule — germ cells built into the wall.

A gonad. Four rows of germ cells in the wall itself, getting smaller toward the lumen, and no stereocilia anywhere.

Examine it yourself

The virtual slide

Zoom to about ×10 and centre one lumen. Look at the apical surface first and satisfy yourself the tufts are not cilia, then count how many levels the nuclei sit at, then look at how little muscle there is outside.

Virtual slide courtesy of Histology Guide.

YOur missions

Field Notes for Future Missions

Mission 1: find the stereocilia

Mission 2: quick check

Field notes

Why this organism

A horsehair worm spends most of its life as a parasite and emerges as a metre of thread that ties itself into a knot you could hold in your palm. The epididymis is the same joke: six metres of duct packed into something you would not notice. Both solve “I need an enormous length and almost no room” the same way, and both look, to the naked eye, like far less than they are.

Next · Pl. III

Vehens muscularis

The Pinhole Cannon · ductus deferens.

In the wild  ·  photograph to come.

List of terms